anti mouse pd 189 l1  (Bio X Cell)

Journal: Parasites & vectors

Article Title: High PD-1 and CTLA-4 expression correlates with host immune suppression in patients and a mouse model infected with Echinococcus multilocularis.

doi: 10.1186/s13071-024-06511-2

Figure Lengend Snippet: Fig. 6 Enhanced ability of Echinococcus multilocularis-infected mouse splenocytes to kill PSCs caused by PD-1/PD-L1 or CTLA-4 blockade. (A) PSCs cultured in vitro under a light microscope in four groups (stained with 0.1% eosin, 100 × magnification). (B) Statistical comparisons of PSC viability among all groups. “PSCs” represents protoscoleces; “N-S” represents splenocytes from uninfected control mice (n = 6); “Em-S” represents splenocytes from E. multilocularis-infected mice (n = 6); “Isotype” represents isotype control antibody; “PD-L1” represents PD-L1 antibody; “CTLA-4” represents CTLA-4 antibody. The yellow arrow indicates dead PSCs completely stained red. The green arrow indicates unstained PSCs with high viability. Data were analyzed using one-way ANOVA plus Tukey’s multiple comparison test. All data are presented as the mean ± standard deviation and are representative of three independent experiments. **p < 0.01; ****p < 0.0001; ns, p > 0.05. Abbreviations: E. multilocularis/Em, Echinococcus multilocularis; PSCs, protoscoleces; ANOVA, analysis of variance

Article Snippet: In addition, an anti-PD-L1 antibody (4 μg/ ml, endotoxin < 0.001 EU/μg, BioXcell, CP168), antiCTLA-4 antibody (4 μg/ml, endotoxin < 0.001 EU/μg, BioXcell, CP146) or isotype control antibody (4 μg/ml, endotoxin < 0.001 EU/μg, BioXcell, BP0083) was added to the co-culture system of PSCs and splenocytes from mice.

Techniques: Infection, Cell Culture, In Vitro, Light Microscopy, Staining, Control, Comparison, Standard Deviation